PREreviews de Non-canonical nuclear function of glutaminase cooperates with Wnt signaling to drive EMT during neural crest development

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Non-canonical nuclear function of glutaminase cooperates with Wnt signaling to drive EMT during neural crest development

par Nioosha Nekooie Marnany, Alwyn Dady, Frédéric Relaix, Roberto Motterlini, Roberta Foresti, Sylvie Dufour et Jean-Loup Duband

Publié: 19 avril 2024
Serveur de preprints: bioRxiv
DOI: 10.1101/2024.04.17.589887

Résumé

Metabolic reprograming has been linked to epithelial-to-mesenchymal transition (EMT) in cancer cells, but how it influences EMT in normal cells remains largely unknown. Here we explored how metabolism impacts delamination and migration of avian trunk neural crest cells, an important stem cell population of the vertebrate embryo. We report that delamination exhibits a quiescent metabolic phenotype whereas migration is characterized by OXPHOS-driven metabolism coupled to distinct expression of metabolic, EMT and developmental genes. While glucose and glutamine are required for delamination and migration, we uncover a novel role for glutamine and its catabolizing enzyme glutaminase in delamination. Specifically, glutamine is required for nuclear translocation of glutaminase, which interacts and cooperates with Wnt signaling to regulate EMT gene expression and cell cycle during delamination. Our data indicate that similarly to cancer cells, embryonic cells engage metabolic enzymes for non-canonical signaling functions to connect metabolism with EMT.

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PREreview par Chad Sansing et al.

Rédigé par Chad Sansing et 1 autre auteur

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PREreview par Blue Sheep et al.

Rédigé par Blue Sheep et Chris Wilkinson

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PREreviews de Non-canonical nuclear function of glutaminase cooperates with Wnt signaling to drive EMT during neural crest development

2 PREreviews

Competing interests